Contact an Account ManagerDetermination of 6-beta-hydroxy-testosterone and 7-α-hydroxy-testosterone from cytochrome P450 on NUCLEODUR® 1.8 µm
Summary
Type: HPLC
Application #: 122140
Catalog#: 760405.20 - MN HPLC Column, Nucleodur C18 Isis, 110A, 1.8um, 2x50mm
Detection:
Conditions: Eluent A: water + 0.1 % formic acid
Eluent B: acetonitrile – methanol + 0.3 % formic acid
Gradient: 75-60 % A in 1.10 min, 60-0 % A in 0.05 min, 0 % A for 0.85 min, 0-75 % A in 0.05 min, 75 % A for 0.95 min
Flow rate: 0.9 mL/min
Temperature: 70 °C
Authors: Unstated customer, France
Source: Macherey-Nagel
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